Wednesday, December 9, 2009

Protocol for determining the GI of a food

Ideally, it should provide 8 or 10 volunteers and tested 2 to 3 times for both the reference food (glucose) that the food which we seek the IG. If this is impossible, merely by voluntary testing, but each volunteer must provide a value for glucose and the reference food.

Receive volunteers at the hospital the day before the test.
Take the conventional measures (height, weight, blood pressure ...)
Serve a light dinner low GI at a time that ensures that volunteers have fasted for 10 h before the first test.
Usually awakenings take place between 6 and 7: 00 am, test begins at 7 pm 30.
Basal blood sampling with a mini-lancet device or self-biting (Autolet ® Miles Autoclix ® Boehringer). Note that it is advisable to measure insulin and more glucose an index for determining insulin in addition to glycemic index. It is also advisable to eliminate the volunteers whose fasting glucose is 7.0 mmol / l or more.
To facilitate the circulation device, you can ask for volunteers to move his hands under warm water for about 5 minutes before each blood sample.

Then the food (ie the reference food: 50 g glucose is to test the food containing 50 grams of digestible carbohydrates) are used and must be consumed by each volunteer for 15 minutes. The countdown begins when the subject is fed.
It may be useful to meet regularly sensation of hunger on a calibrated ruler for the occasion.
Blood samples were taken 15, 30, 45, 60, 90 and 120 min after the beginning of the "meal".
IG (%) is calculated by dividing the incremental area under the curve (IAUC) of the food tested by the incremental area under the curve of the reference food (IAUC) and multiplying the result by 100.
GI is calculated individually for each volunteer separately from its response to the food tested and its response to the reference food.
If each volunteer was tested repeatedly for the same food and the reference food (which is preferable), it is the average GI by topic.
For the GI of the food, being averaged from the respective averages of each volunteer.
The variability of the GI of each food is measured by standard deviation of the mean.

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